Trihybrid Cross Calculator - Punnett Square

Generate the phenotype ratio for a trihybrid (three-gene) cross.

Parent 1, Gene 1

Parent 1, Gene 2

Parent 1, Gene 3

Parent 2, Gene 1

Parent 2, Gene 2

Parent 2, Gene 3

Result

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How it works

Assumes independent assortment of all three genes (no linkage). Classic heterozygous × heterozygous trihybrid cross gives a 27:9:9:9:3:3:3:1 ratio.